active p60 c src Search Results


active p60-c-src  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc active p60-c-src
Active P60 C Src, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/active p60-c-src/product/Cell Signaling Technology Inc
Average 90 stars, based on 1 article reviews
active p60-c-src - by Bioz Stars, 2026-02
90/100 stars
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catalytically active recombinant p60c-src  (Upstate Biotechnology Inc)
90
Upstate Biotechnology Inc catalytically active recombinant p60c-src
Catalytically Active Recombinant P60c Src, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/catalytically active recombinant p60c-src/product/Upstate Biotechnology Inc
Average 90 stars, based on 1 article reviews
catalytically active recombinant p60c-src - by Bioz Stars, 2026-02
90/100 stars
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p60 c-src protein-tyrosine kinase cat# pk03  (Millipore)
90
Millipore p60 c-src protein-tyrosine kinase cat# pk03
Microtiter plate array peptides serve as substrates for <t>p60</t> c-src with specificity and concentration dependence Eight-well strips bearing six substrate peptide wells and two control wells were robotically synthesized ab initio , in microtiter plate format. All of the peptide substrates were initiated with Cys(Acm) to provide additional extension from the polylysine backbone and a common attachment site. The Cys-SH protecting group (Acm) remains in place and all peptides were acetylated at the amino terminus. Duplicate substrate strips were reacted with each 90-μL dilution of c-Src enzyme containing 3, 1, 0.3, and 0.1 Units of enzyme. The wells were reacted for 20' at 30°, washed with distilled water and assayed by antibody ELISA for the presence of phosphotyrosine using a mixture of antibodies described in Figure 5. Nonlinear regression plots were computed in Prism and error bars represent the means ± 1 SD, which ranged between 3% and 7% of mean values. Iterations for (ABL) did not converge. All data points were corrected by subtraction of values obtained from wells with no peptide.
P60 C Src Protein Tyrosine Kinase Cat# Pk03, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p60 c-src protein-tyrosine kinase cat# pk03/product/Millipore
Average 90 stars, based on 1 article reviews
p60 c-src protein-tyrosine kinase cat# pk03 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
p60c-src  (Millipore)
90
Millipore p60c-src
Microtiter plate array peptides serve as substrates for <t>p60</t> c-src with specificity and concentration dependence Eight-well strips bearing six substrate peptide wells and two control wells were robotically synthesized ab initio , in microtiter plate format. All of the peptide substrates were initiated with Cys(Acm) to provide additional extension from the polylysine backbone and a common attachment site. The Cys-SH protecting group (Acm) remains in place and all peptides were acetylated at the amino terminus. Duplicate substrate strips were reacted with each 90-μL dilution of c-Src enzyme containing 3, 1, 0.3, and 0.1 Units of enzyme. The wells were reacted for 20' at 30°, washed with distilled water and assayed by antibody ELISA for the presence of phosphotyrosine using a mixture of antibodies described in Figure 5. Nonlinear regression plots were computed in Prism and error bars represent the means ± 1 SD, which ranged between 3% and 7% of mean values. Iterations for (ABL) did not converge. All data points were corrected by subtraction of values obtained from wells with no peptide.
P60c Src, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p60c-src/product/Millipore
Average 90 stars, based on 1 article reviews
p60c-src - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


Microtiter plate array peptides serve as substrates for p60 c-src with specificity and concentration dependence Eight-well strips bearing six substrate peptide wells and two control wells were robotically synthesized ab initio , in microtiter plate format. All of the peptide substrates were initiated with Cys(Acm) to provide additional extension from the polylysine backbone and a common attachment site. The Cys-SH protecting group (Acm) remains in place and all peptides were acetylated at the amino terminus. Duplicate substrate strips were reacted with each 90-μL dilution of c-Src enzyme containing 3, 1, 0.3, and 0.1 Units of enzyme. The wells were reacted for 20' at 30°, washed with distilled water and assayed by antibody ELISA for the presence of phosphotyrosine using a mixture of antibodies described in Figure 5. Nonlinear regression plots were computed in Prism and error bars represent the means ± 1 SD, which ranged between 3% and 7% of mean values. Iterations for (ABL) did not converge. All data points were corrected by subtraction of values obtained from wells with no peptide.

Journal: BMC Immunology

Article Title: Fully automated synthesis of (phospho)peptide arrays in microtiter plate wells provides efficient access to protein tyrosine kinase characterization

doi: 10.1186/1471-2172-6-1

Figure Lengend Snippet: Microtiter plate array peptides serve as substrates for p60 c-src with specificity and concentration dependence Eight-well strips bearing six substrate peptide wells and two control wells were robotically synthesized ab initio , in microtiter plate format. All of the peptide substrates were initiated with Cys(Acm) to provide additional extension from the polylysine backbone and a common attachment site. The Cys-SH protecting group (Acm) remains in place and all peptides were acetylated at the amino terminus. Duplicate substrate strips were reacted with each 90-μL dilution of c-Src enzyme containing 3, 1, 0.3, and 0.1 Units of enzyme. The wells were reacted for 20' at 30°, washed with distilled water and assayed by antibody ELISA for the presence of phosphotyrosine using a mixture of antibodies described in Figure 5. Nonlinear regression plots were computed in Prism and error bars represent the means ± 1 SD, which ranged between 3% and 7% of mean values. Iterations for (ABL) did not converge. All data points were corrected by subtraction of values obtained from wells with no peptide.

Article Snippet: Peptide array substrate evaluations were performed using p60 c-src protein-tyrosine kinase, Cat# PK03 (Oncogene Research Products) according to the instructions in the manufacturer's insert.

Techniques: Concentration Assay, Control, Synthesized, Enzyme-linked Immunosorbent Assay